Figure 1.
RNAi effectively blocks the expression of lhx6 in GE neurons. A-D, Dissociated MGE cells taken from E13 mice were transfected with pEGFP and control vector (A, B) or pEGFP and the siRNA vector (C, D) and immunostained for Lhx6 (red). A high proportion of MGE cells transfected with pEGFP/control vector were also positive for Lhx6 (A, B, arrows). Dissociated MGE cells transfected with pEGFP/siRNA vector did not exhibit Lhx6 immunoreactivity, indicating that the production of RNAi in these cells inhibited lhx6 expression (n = 5 experiments). E, F, siRNA transfection did not affect cell viability as shown with DAPI nuclear staining (arrows). Scale bars, 50 μm.