Figure 5.
Synaptic localization of GluRIIA is decreased in dock mutants. A, Representative images of a wild-type (left) and a dock null NMJ [dockP1/Df(2L)ast2] stained with anti-GluRIIA, anti-Pak, and anti-HRP. GluRIIA staining is reduced in the dock mutant animals without a corresponding decrease in Pak levels at the synapse. B, Quantification of the fluorescence intensity of anti-GluRIIA, anti-HRP, and anti-PAK staining. There is a significant decrease in the intensity of GluRIIA staining in dockP1/Df(2L)ast2 null animals (61.2 ± 3.6%; n = 8) and dock4/Df(2L)ast2 (64.3 ± 4.8%; n = 6) compared with wild-type and the heterozygous controls dockP1/+, dock4/+, and Df(2L)ast2/+. Additionally, we observed a significant increase in GluRIIA staining and Pak staining in the dockP1/+ animals (*p < 0.05; **p < 0.0002).