Figure 5.
Synaptic accumulation of GFP—Shank1B depends on interaction between Homer and Shank. A—E, Neurons were cotransfected with GFP—Shank1B plus empty vector (A), Homer1a (B), Homer1b (C), Homer1aW24A (D), or Shank1B1440—1849 (E). F—H, Neurons were cotransfected with GFP—Shank1–1440 plus empty vector (F), Homer1a (G), or Homer1b (H). I—K, Neurons were cotransfected with GFP—Homer1b plus empty vector (I), Homer1a (J), or Homer1aW24A (K). A—K show images of dendrites from transfected neurons in the GFP channel. L, Synaptic targeting of GFP—Shank1B, GFP—Shank1B1–1440, and GFP—Homer1b in neurons transfected with the indicated constructs, quantified by the ratio of staining intensity in spine/shaft (>10 neurons examined for each construct, corresponding to at least 6000 μm of dendrite length and 1800 synapses). Error bars indicate SEM. *p < 0.01. Scale bar, 10 μm.