Figure 2.
T cells infiltrate the lesion-reactive hippocampus. Flow cytometry profiles show live cells on the basis of forward and side scatter gating. Shown are representative profiles from individual unmanipulated (U, left column), contralateral (C, middle column), or lesion-reactive (L, right column) hippocampi from C57BL/6 (A), CCR2-deficient (B), or CCR5-deficient (C) mice at 24 hr after axotomy. T cells were identified on the basis of expression of CD3 and CD45 (box). D shows T cell proportions within groups of hippocampi between 3 hr and 14 d after axotomy (mean ± SE). Boxes were drawn on the basis of fluorescence levels using isotype-matched control antibodies. Data are representative of three to seven animals per group. *p < 0.05 versus U and C; **p < 0.05-0.001 versus U, C, and L of C57BL/6 at 3, 12, and 24 hr.