Figure 2.
Extinction training-induced calcineurin expression is blocked by anisomycin but not by actinomycin D. A, Behavioral procedure used in experiment B. Extinction-trained rats were killed by decapitation immediately after light-alone trials. The LA and BLA were microdissected, and cytosolic calcineurin levels were measured as described in Materials and Methods. B, Calcineurin levels in the extinction-trained rats given drugs or vehicle were normalized to those of unpaired controls. Light-alone trials induced an increased expression of calcineurin in cytosolic (α-actin as internal control) fraction that could be blocked by anisomycin (125 μg dissolved in 1.6 μl of vehicle; 0.8 μl per side). In contrast, actinomycin D (10 μg dissolved in 1.6 μl of vehicle; 0.8 μl per side) was without effect. *p < 0.01 versus vehicle. C, Cannula tip placements from paired rats infused with vehicle (open circle), anisomycin (filled circle), or actinomycin D (filled triangle).