Figure 6.
Phenotype of donor-derived cells with a rod-shaped morphology. a–d, Confocal images of a GFP-positive rod cell (green, arrows) that was associated with a microvessel (red) and did not express Iba1 (blue). e–h, Two contiguous rod cells (arrows) that stained positively for CD45. i–m, A rod cell located inside a blood vessel. m, This reconstructed image corresponding to an x–z-plane of i (dotted line) shows that the GFP-labeled cell is located within the microvessel. n–p, Rod cells of different sizes exhibiting characteristic morphology. q–v, Rod cells showing apparent sprouts of variable length and position. w, Rod cell doublet. x, Quantification indicates the proportion of rod-shaped cells that appeared as sprouting cells or doublets versus the population of individual (singly occurring–nonsprouting) rod cells did not change significantly between 3 and 6 months after transplantation. y, A hypothetical model for rod cell development in the adult mouse brain. Scale bar: a–w, 5 μm.