Table 1.
Mean and SE of 5-HT-positive cells and single c-Fos-positive nuclei in different regions of the DRN of sedentary and physically active rats exposed to either uncontrollable tail shock (stressed) or control treatment
Dorsal raphe nucleus region | Sedentary control (n = 6) | Sedentary stressed (n = 6) | Physically active control (n = 6) | Physically active stressed (n = 7) | ||||
---|---|---|---|---|---|---|---|---|
Mean | SE | Mean | SE | Mean | SE | Mean | SE | |
5-HT-positive cells | ||||||||
Dorsal DRN | ||||||||
Rostral | 76.0 | 4.03 | 75.83 | 8.66 | 73.17 | 6.66 | 72.43 | 8.69 |
Mid | 105.67 | 10.53 | 98.66 | 7.68 | 98.17 | 10.09 | 101.14 | 8.88 |
Caudal | 72.83 | 4.0 | 75.83 | 7.98 | 64.33 | 4.06 | 79.0 | 5.2 |
Ventral DRN | ||||||||
Rostral | 62.83 | 3.27 | 64.83 | 3.86 | 68.33 | 3.58 | 70.43 | 6.24 |
Mid | 73.17 | 5.72 | 79.33 | 1.75 | 81.5 | 4.82 | 72.28 | 4.99 |
Caudal | 51.5 | 2.5 | 52.17 | 3.27 | 51.0 | 1.67 | 45.0 | 3.16 |
Lateral DRN | ||||||||
Rostral | 7.67 | 1.99 | 9.17 | 2.27 | 8.67 | 1.31 | 9.86 | 1.68 |
Mid | 63.33 | 3.24 | 44.83 | 3.72 | 53.17 | 4.16 | 51.67 | 5.21 |
Single c-Fos-positive nuclei | ||||||||
Dorsal DRN | ||||||||
Rostral‡,1-253§ | 0.33 | 0.21 | 49.83** | 6.07 | 0.50 | 0.34 | 31.0**† | 3.82 |
Mid‡ | 0.17 | 0.17 | 24.33** | 4.24 | 0.33 | 0.21 | 24.43** | 2.75 |
Caudal‡ | 0.67 | 0.33 | 6.83** | 0.79 | 0.50 | 0.34 | 7.43** | 1.23 |
Ventral DRN | ||||||||
Rostral‡ | 0.33 | 0.21 | 12.83** | 1.54 | 0.50 | 0.22 | 8.86** | 2.94 |
Mid‡ | 0.50 | 0.22 | 9.67** | 3.31 | 0.17 | 0.17 | 9.0** | 1.40 |
Caudal‡ | 0.33 | 0.42 | 4.0** | 0.82 | 0.67 | 0.49 | 3.86** | 0.91 |
Lateral DRN | ||||||||
Rostral‡ | 0.0 | 0.0 | 46.67** | 7.75 | 0.17 | 0.17 | 43.71** | 6.42 |
Mid‡ | 0.17 | 0.17 | 43.16** | 4.55 | 0.0 | 0.0 | 46.5** | 5.18 |
Two-way ANOVA:
main effect of stress,p < 0.001;
F1-253: main effect of activity,p < 0.05; §stress by activity interaction, p < 0.05. Fisher protected least significant difference: **p < 0.001 versus control;
p < 0.05 versus sedentary stressed.