Fig. 10.
NMDA receptor activation regulates dendritic spine localization of PKA and CaN with AKAP79/150.A, NMDA receptor regulation of PKA-RIIβ and AKAP79/150 localization. B, Disruption of dendritic spine colocalization of CaNB and AKAP79/150 in response to NMDA receptor activation. Neurons (2–3 weeks old) were untreated, pretreated with antagonist AP-V (100 μm, 30 min), or treated with glutamate (50 μm, 10 min) as indicated. Control untreated and treated neurons were stained for AKAP79/150 (red) and either PKA-RIIβ regulatory subunits (green) in A or CaNB (green) inB. The smaller panels are magnifications of dendrites. See Results for quantitation.