Fig. 4.
Butyrolactone I prevents stress-induced facilitation of context-dependent fear conditioning. A, Immunoblot of septohippocampal lysates (30 μg/lane) stained with antibodies to Cdk1, Cdk2, and Cdk5. Note the positive signal for Cdk5 in mice stressed for 1 hr and killed 0.5 hr later, but not for Cdk1 or Cdk2 in naive or identically stressed mice. Positive controls consisted of spleen cell lysate (30 μg/lane) and epidermal growth factor-stimulated A431 cell lysate (20 μg/lane). B, Experimental design. C, Effect of butyrolactone I injected into the intermediate septum before stress and fear conditioning on freezing during the context-dependent memory test. Significant group differences (F(3,30)= 17.14; p < 0.001) were observed.D, Effect of butyrolactone injected into the mediodorsal hippocampus before stress and fear conditioning on freezing during the context-dependent memory test. Significant group differences (F(3,35) = 8.673; p< 0.001) were observed. Post hoc analyses: *p < 0.05 versus nonstressed vehicle group; ***p < 0.01 versus nonstressed vehicle group;‡‡‡p < 0.001, stressed butyrolactone I group versus stressed vehicle group.