Fig. 2.
A, MTase protein levels in olfactory bulb (OB), striatum (STR), and hippocampus (HIP) in brain lysates from normal (wt) and DnmtS/+ mice (s/+). Brain lysates were subjected to SDS-PAGE and immunoblot analysis performed using a polyclonal MTase antibody. The experiment was repeated three times; a representative experiment is shown. B, Time-dependent changes in MTase protein expression in striatum during reperfusion after 30 min middle cerebral artery occlusion. MTase protein was present in normal brain (sham) and did not change over time (0, 1, 3, 6, 12, 18, 24, 48, and 72 hr) between left (L = ischemic) and right (R = non-ischemic) hemispheres. The experiment was repeated three to four times per time point; a representative experiment for the time points 3, 6, and 18 hr is shown.