Table 1.
Markers expressed by freshly isolated cells or after 36 hr in culture (%)
| Lineage | Marker | Hippocampus | Cortex | Optic nerve | |||
|---|---|---|---|---|---|---|---|
| Lower 0 hr | Lower 36 hr | Upper 36 hr | Lower 0 hr | Lower 36 hr | Lower 36 hr | ||
| Progenitor | Nestin | 15 | 0.1 | <0.1 | 7 | <0.1 | <0.1 |
| A2B5 | 1 | 0.5 | nd | 0.8 | 0.7 | 1.2 | |
| O4 | 12 | 24 | 2 | 15 | 29** | 31 | |
| Vimentin* | 37 (34) | 87 (36) | 68 (15) | 35 (30) | 89 (15) | 76 (18) | |
| Glia | GFAP | <0.1 | 0.1 | 26 | <0.1 | <0.1 | <0.1 |
| RIP | <0.1 | <0.1 | 14 | <0.1 | <0.1 | <0.1 | |
| GalC | nd | <0.1 | 12 | <0.1 | <0.1 | <0.1 | |
| Neuron | βTubulin | 0.1 | 0.1 | 9 | <0.1 | <0.1 | <0.1 |
| Map2 | <0.1 | <0.1 | 18 | <0.1 | <0.1 | <0.1 | |
| NeuN | nd | <0.1 | 15 | <0.1 | <0.1 | <0.1 | |
| Other | Fibronectin | <0.1 | <0.1 | 2 | <0.1 | <0.1 | nd |
| Ox42 | <0.1 | <0.1 | 1 | <0.1 | <0.1 | nd | |
| ED-1 | <0.1 | <0.1 | <0.1 | <0.1 | nd | nd | |
| BrdU | 0.7 | 0.8 | <0.01 | 0.1 | 0.2 | nd | |
*Total immunoreactive cells versus acid-stable immunoreactive cells; nd, not determined; means are reported for three to seven separate cell isolates; **O4 immunoreactivity in 36 hr low-buoyancy cortical cultures showed the largest relative SEM and was 14% of the mean or 29 ± 4. Map2, Microtubule-associated protein 2; RIP, receptor-interacting protein. NeuN, Neuronal nuclear antigen; ED-1, microglial marker; GalC, galactocerebroside.