Table 3.
Cellular localization of huntingtin in striatum reported by various authors3-a
| Authors of study | Amino acid sequence target of antisera3-b | Species studied | Cholinergic interneurons | Parvalbumin interneurons | SS/NPY/NOS interneurons | Calbindinergic projection neurons | Patch vs matrix |
|---|---|---|---|---|---|---|---|
| Present paper | aa181–812 aa1247–1646 aa2416–2541 | Rat | Rich in Ht | Minority rich in Ht, majority poor in Ht | Vast majority devoid of Ht | 65% moderate in Ht; remainder devoid of Ht | Patch same as matrix |
| Kosinski et al., 1997 | aa1981–2580 | Rat | Poor in Ht | Minority rich in Ht, majority poor in Ht | Vast majority devoid of Ht | Approximately 90% moderate to rich in Ht; remainder devoid of Ht | Patch richer than matrix |
| Ferrante et al., 1997 | aa549–679 | Human | Poor in or devoid of Ht | Not done | Devoid of Ht | Approximately half moderate in Ht; remainder devoid of Ht | Patch poorer than matrix |
| Sapp et al., 1997 | aa585–745 | Human | Large neurons labeled for Ht; unclear how intensely | Not done | Not done | Many contain Ht; unclear how many or how intensely | Patch poorer than matrix |
| Bhide et al., 1996 | aa1–17 | Mouse | Presumptive cholinergic neurons rich in Ht | Not done | Not done | Many or all moderate in Ht | Patch same as matrix |
| Gutekunst et al., 1995 | aa549–679 | Rat, monkey, humans | Presumptive cholinergic neurons rich in Ht | Not done | Not done | Many or all moderate in Ht | Presumed patch richer than matrix in human |
F3-a
This table compares the findings of the present study and previous immunohistochemical studies on the localization of huntingtin (Ht) in the striatal neuron types examined in the present study.
F3-b
Although the studies noted in this table in many cases used a number of different antisera against Ht, only the antisera indicated were used for the localization of Ht in the neuronal cell types of relevance to this table.