Fig. 4.
Cellular localization of rREST mRNA in the adult rat brain by in situ hybridization. Shown are bright-field emulsion autoradiographs obtained after hybridization of coronal sections of adult rat brain with the [α-35S]-labeled rREST cRNA probe corresponding to the region upstream of zinc finger 9 (riboprobe 11; see Materials and Methods and Fig. 1B). Shown is rREST-specific labeling in the cells of the following: A, granular layer of olfactory bulb; B, dentate gyrus of hippocampus; C, cerebral cortex; D, CA1 pyramidal layer of hippocampus; E, along the border between the granular and molecular layers of the cerebellar cortex;F, gigantocellular reticular nucleus; andG, substantia nigra pars compacta. A section hybridized with the sense RNA probe is shown in H, corresponding to the brain area shown in F. Exposure time was 6 weeks.Arrows point to dense accumulations of silver grains over individual cells. Scale bar, 12 μm.