Fig. 7.
Southern blot analysis of rREST gene. Rat genomic DNA was digested with the indicated restriction enzymes. Two identical filters were hybridized with probes corresponding to different regions of rREST cDNA. Left, Hybridization with the 460 bpPvuII/AvaII rREST cDNA fragment (riboprobe 4; see Materials and Methods) spanning the region encoding part of the N terminus up to the zinc finger 2 motif.Right, Hybridization with the 360 bpAvaII/DraI rREST cDNA fragment (riboprobe 5; see Materials and Methods) covering the region from the zinc finger 2 motif up to the zinc finger 6 motif. The detection of two genomic DNA fragments of different sizes in both lanes of the right panel indicates that at least one intron is present in the region encoding the N-terminal zinc finger cluster. The DNA molecular weight size markers (in kb) are indicated at left.