Fig. 9.
Expression of rREST mRNA in the hippocampus after kainic acid treatment. A, RPA of rREST1, rREST4, and rREST mRNA expression in the hippocampus at 2, 4, and 24 hr after KA-induced seizures. The amount of RNA used in RPA was 20 μg for rREST1 transcripts (riboprobe 6; see Materials and Methods and Fig.1B) and 40 μg for rREST4 transcripts (riboprobe 9; see Materials and Methods and Fig. 1B). Total RNA was isolated from the hippocampus of saline- or KA-treated animals. Protected fragments corresponding to rREST1, rREST4, rREST, and GAPDH mRNA are indicated. Saline treatment was performed for 2, 4, and 24 hr; no changes were detected in the expression levels of any of the rREST transcripts. B, Dark-field autoradiographs showing rREST mRNA-specific labeling in the adult rat brain at 4 and 24 hr after KA-induced seizures. Coronal sections were prepared at the level of dorsal hippocampus and hybridized with the rREST-specific cRNA probes (riboprobes 4 and 11; see Materials and Methods and Fig.1B). contr., Endogenous levels of corresponding rREST transcripts in hippocampus; saline,levels of rREST1, rREST4, and rREST transcripts 4 hr after saline treatment; KA, kainic acid; tRNA, yeast tRNA as a negative control; SENSE, section hybridized with the [α-35S]-labeled sense riboprobe;CP, choroid plexus; dg, dentate gyrus of the hippocampus; CTX, cerebral cortex;CA1, CA3, CA4, pyramidal layers CA1, CA3, and CA4 of the hippocampus.