Fig. 5.
Clonal generation of neurospheres from subependymal neural stem cells in vitro is not affected in TGFα-deficient and senescent mice. The number of neurospheres generated per brain from (A) adult TGFα(−/−) mice and B6129/F2 controls and (B) 23- to 25-months-old and 2- to 4-months-old controls after 12–14 d in vitro. Tissue from the medial and lateral (including the dorsolateral corner) aspects of the subependyma was dissected from both hemispheres, pooled together, and cultured in serum-free media in the presence of 20 ng/ml EGF. Data represent the mean ± SEM for independent culture preparations from three mice in each group.