Fig 1.
Dose-escalation study. Irradiation was 4 hours post-fertilization (4hpf). Eggs were given 5–12 Gy delivered with FLASH or conventional dose rate irradiation. Radiation-induced alteration of zebrafish morphology was assessed 5 days post-fertilization (5dpf) by body length measurement. FLASH radiotherapy induced lower morphological alterations than conventional radiotherapy at doses above 10 Gy. Mean ± standard deviation and Mann–Whitney’s-test: *P < 0.05; **P < 0.01; ***P < 0.001 (n = 9–19 embryos/group).