Table 4.
ELISA linearity-of-dilution results for the tested RBC sample.
Dilution factor (DF) | Observed (μg/ml) × DF | Expected (μg/ml, neat value) | Recovery (%) |
---|---|---|---|
Neat | 2.6 | 2.6 | 100 |
1 : 2 | 3.1 | 119 | |
1 : 4 | 3.3 | 127 | |
1 : 8 | 3.6 | 138 |
Dilutions were made in a previously chosen sample diluent. The observed value was assessed relative to the assay standard curve.