KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit α-Ku80 (Xenopus) | Graham et al., 2016 | N/A |
| Rabbit α-XLF (Xenopus) | Graham et al., 2016 | N/A |
| Rabbit α-XRCC4 (Xenopus) | Graham et al., 2016 | N/A |
| Rabbit α-DNA-PKcs (Xenopus) | Graham et al., 2016 | N/A |
| Rabbit α-ORC2 (Xenopus) | Walter and Newport, 1997 | N/A |
| Rabbit α-Tdp1 (Xenopus) | This study | N/A |
| Rabbit α-Artemis (Xenopus) | This study | N/A |
| Rabbit α-PNKP (Xenopus) | This study | N/A |
| Rabbit α-pol λ (Xenopus) | This study | N/A |
| Rabbit α-pol μ (Xenopus) | This study | N/A |
| Goat α-rabbit IgG, horseradish peroxidase-conjugated | Jackson ImmunoResearch | Cat# 111-035-003 |
| Bacterial and Virus Strains | ||
| Rosetta 2(DE3) E. coli | Novagen | Cat# 71400 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Sulfo-Cyanine3 NHS ester | Lumiprobe | Cat# 11320 |
| Sulfo-Cyanine5 NHS ester | Lumiprobe | Cat# 13320 |
| Cy3B NHS ester | GE Healthcare | Cat# PA63101 |
| ATTO 647N NHS ester | ATTO-TEC | Cat# AD 647-31 |
| NU7441 | R&D Systems, Inc. | Cat# 3712 |
| γ−32P-ATP | Perkin Elmer | Cat# BLU502A250UC |
| Amino-11-dUTP | Lumiprobe | Cat# 26040 |
| BHQ-10 succinimidyl ester | Biosearch Technologies | Cat# BHQ-10S-5 |
| Xenopus laevis Lig4-XRCC4 | This study | N/A |
| Xenopus laevis PNKP | This study | N/A |
| Xenopus laevis pol λ | This study | N/A |
| Xenopus laevis pol μ | This study | N/A |
| Xenopus laevis Tdp1 | This study | N/A |
| Protein A sepharose Fast Flow | GE Healthcare | Cat# 17127903 |
| AminoLink Coupling Resin | Thermo Scientific | Cat# 20382 |
| Nocodazole | Sigma | Cat# M1404 |
| mPEG-SVA, MW 5,000 | Laysan Bio | Cat# MPEG-SVA-5K-1g |
| Biotin-PEG-SVA, MW 5,000 | Laysan Bio | Cat# Bio-PEG-SVA-5k-100mg |
| Streptavidin | Sigma | Cat# S4762 |
| Protocatechuic acid | Sigma | Cat# 37580 |
| Protocatechuate 3,4-dioxygenase | Sigma | Cat# P8279 |
| Trolox | Sigma | Cat# 238813 |
| Streptavidin-coupled magnetic Dynabeads M-280 | Invitrogen | Cat# 11206D |
| Experimental Models: Organisms/Strains | ||
| Xenopus laevis, adult female | Nasco | Cat# LM00535 |
| Oligonucleotides | ||
| Please see Table S1. | ||
| Recombinant DNA | ||
| pBMS6 | This study | N/A |
| pBMS10 (PNKP expression) | This study | N/A |
| pBMS49 (L4WTX4 expression) | This study | N/A |
| pBMS50 (L4ciX4 expression) | This study | N/A |
| pBMS14 (pol μ expression) | This study | N/A |
| pBMS25 (pol λ expression) | This study | N/A |
| pBMS60 (Tdp1 expression) | This study | N/A |
| Software and Algorithms | ||
| MATLAB | Mathworks | https://www.mathworks.com/products/matlab.html |
| HCImage Live | Hamamatsu | https://hcimage.com/hcimage-overview/hcimage-live/ |
| iSMS | Preus et al., 2015 | http://inano.au.dk/about/research-groups/single-molecule-biophotonics-group-victoria-birkedal/software/ |
| ebFRET | van de Meent et al., 2014 | http://ebfret.github.io/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/index.html |
| Other | ||
| High Speed Supernatant (HSS) | Lebofsky et al., 2009 | |