Fig. 2.
Quantification of Pseudomonas aeruginosa by real-time LightCycler PCR using standard curve analysis. In the upper part of the diagram, fluorescence signals of a 1:10 dilution series of defined P. aeruginosa genome equivalents (1 × 108 to 1 × 102) are displayed (measurement in duplicates). Based on the correlation of crossing-point CP (PCR cycle which is significantly different to the background fluorescence noise) to P. aeruginosa DNA concentration, a standard curve was calculated (lower part of the diagram). The linear correlation of this standard curve (theoretical slope = −3.33; determined slope = −3.86) allows the quantification of an unknown sample based on the CP of the respective sample