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. 1977;55(1):77–86. doi: 10.1007/BF01314481

Detection of transmissible gastroenteritis virus neutralising antibody in cats

D J Reynolds 1, D J Garwes 1, C J Gaskell 2
PMCID: PMC7087289  PMID: 200203

Summary

High titres of neutralising activity to transmissible gastroenteritis virus (TGEV), a porcine coronavirus, were found in sera and peritoneal fluids from cats infected with feline infectious peritonitis (FIP). A small proportion of cats, from a hospital population unaffected by FIP, also had neutralising activity. Procedures to remove non-specific viral inhibitors, including treatment by heat inactivation, trypsin, sulphydryl reagent and kaolin absorption were unsuccessful. The active component was unable to neutralise another porcine coronavirus, haemagglutinating encephalomyelitis virus or the porcine enterovirus, Talfan. Gel filtration of feline sera and peritoneal fluid demonstrated high levels of the neutralising activity in the area corresponding to 7S IgG, which could be removed by absorption with specific anti-IgG serum and these properties are suggested to be consistent with those of antibody. These findings imply that there is a coronavirus in cats which is antigenically related to TGEV and its possible nature is discussed.

Keywords: Trypsin, Peritonitis, Kaolin, High Titre, Gastroenteritis

Footnotes

With 4 Figures

References

  • 1.Binn, L. N., Lazar, E. C., Keenan, K. P., Huxsoll, D. L., Marchwicki, R. H., Strano, A. J.: Recovery and characterization of a coronavirus from military dogs with diarrhoea. In: Proceedings 78th Ann. meeting, U.S. Anim. Health Assoc. Roanoke, Va., Oct. 1974. 359–366 (1975). [PubMed]
  • 2.Cartwright S., Lucas M. Vomiting and diarrhoea in dogs. Vet. Rec. 1972;91:571–572. doi: 10.1136/vr.91.23.571. [DOI] [PubMed] [Google Scholar]
  • 3.Doggett J. E., Taylor-Robinson D., Gallop R. G. C. A study of an inhibitor in bovine serum active against respiratory syncytial virus. Arch. ges. Virusforsch. 1968;23:126–137. doi: 10.1007/BF01242120. [DOI] [PubMed] [Google Scholar]
  • 4.Gouffaux M., Pastoret P. P., Henrcteaux M., Massip A. Feline infectious peritonitis. Proteins of plasma and ascitic fluids. Vet. Pathol. 1975;12:335–348. doi: 10.1177/0300985875012005-00601. [DOI] [PubMed] [Google Scholar]
  • 5.Haelterman E. O. Epidemiological studies of transmissible gastroenteritis of swine. Proc. U.S. Livestock Sanit. Assn. 1962;66:305–315. [Google Scholar]
  • 6.Horzinek, M. C., Osterhaus, A. D. M. E., Ellens, D. J.: Feline infectious peritonitis virus. I. Purification and electron microscopy. Z. Vet. Med. (In press.) [DOI] [PMC free article] [PubMed]
  • 7.Joo H. S., Donaldson-Wood C. R., Johnson R. H. A microneutralization test for the assay of porcine parvovirus antibody. Arch. Virol. 1975;47:337–341. doi: 10.1007/BF01347974. [DOI] [PubMed] [Google Scholar]
  • 8.Kärber G. Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch. exp. path. Pharmakol. 1931;162:480–483. [Google Scholar]
  • 9.Lukert P. D. Avian infectious bronchitis virus. Characteristics of an inhibitor found in serum. Arch. ges. Virusforsch. 1973;40:93–104. doi: 10.1007/BF01242641. [DOI] [PubMed] [Google Scholar]
  • 10.McClurkin A. W., Stark, Sharon L., Norman J. O. Transmissible gastroenteritis (TGE) of swine: The possible role of dogs in the epizootiology of TGE. Canad. J. comp. Med. 1970;34:347–349. [PMC free article] [PubMed] [Google Scholar]
  • 11.Pedersen N. C. Morphologic and physical characteristics of feline infectious peritonitis and its growth in autochthonous peritoneal cell cultures. Amer. J. vet. Res. 1976;37:567–572. [PubMed] [Google Scholar]
  • 12.Pedersen N. C. Serologic studies of naturally occurring feline infectious peritonitis. Amer. J. vet. Res. 1976;37:1449–1453. [PubMed] [Google Scholar]
  • 13.Pocock, D. H., Garwes, D. J.: The polypeptides of haemagglutinating encephalomyelitis virus and isolated subviral particles. (In preparation.)
  • 14.Rutter J. M., Luther P. D. Cytopathic factors in bacteria-free lysates ofEscherichia coli. J. med. Microbiol. 1973;6:565–571. doi: 10.1099/00222615-6-4-565. [DOI] [PubMed] [Google Scholar]
  • 15.Saif L. J., Bohl E. H., Gupta R. K. P. Isolation of porcine immunoglobulins and determination of the immunoglobulin class. Infect. Immun. 1972;6:600–609. doi: 10.1128/iai.6.4.600-609.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Stone S. S., Kemeny L. J., Jensen M. T. Partial characterization of the principal soluble antigens associated with the coronavirus of transmissible gastroenteritis by complement fixation and immunodiffusion. Infect. Immun. 1976;13:521–526. doi: 10.1128/iai.13.2.521-526.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Tyrrell D. A. J., Almeida J. D., Cunningham C. H., Dowdle W. R., Hofstad M. S., McIntosh K., Tajima M., Zakstelskaya L. Ya., Easterday B. C., Kapikian A., Bingham R. W. Coronaviridae. Intervirology. 1975;5:76–82. doi: 10.1159/000149883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Witte K. H. Micro-color test for assay of TGE virus-neutralizing antibodies. Arch. ges. Virusforsch. 1971;33:171–176. doi: 10.1007/BF01254174. [DOI] [PubMed] [Google Scholar]

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