Figure 1.
Concept of studying virus–host interactions by large-scale high throughput methods. Y2H is used for initial screening of viral orfeomes (cloned virus ORFs) against human cDNA libraries expressing human genes. Positive protein interaction pairs are validated in mammalian cells by various methods including (modified) LUMIER, PCA, F2H, etc. Crystal structure analysis is performed on especially interesting protein interaction complexes. Bioinformatic analysis of cellular interaction partners of viral proteins aims at the identification of viral and cellular proteins and signaling pathways as targets for antiviral therapy.