Table 3.
Summary of test costs, hands-on time and total extraction time among the extraction kits
| Parameters | IsoQuick | MinElute Spin | RNAzol B | MinElute Vacuum |
|---|---|---|---|---|
| Costs/test (US$) | 2.86 | 4.69 | 4.42 | 4.54 |
| Labor cost saved per testa (US$) | Not applicable | 0.70 | Not applicable | 2.50 |
| Hands-on time (min)b | 46.1 ± 4.8c | 30.4 ± 2.6 | 36.2 ± 4.4 | 36.1 ± 3.0 |
| Total extraction time (min)b | 124.5 ± 6.6 | 49.0 ± 3.5 | 122.1 ± 8.2 | 54.6 ± 3.9 |
a
Based on a batch size of 10 samples and an hourly wage of US$ 20.00.
b
Hands-on time and total extraction time were estimated from four full runs with two technologists run on two different days.
c
Mean ± S.D. There was no statistical significance in hands-on time among the kits. Total extraction time for IsoQuick and/or RNAzol B was significantly longer than the MinElute.