Figure 5.
Phylogenetic analysis of partial 1a and partial spike gene sequences of HCoV‐NL63 isolates. HCoV‐229E was used to root the tree for the 1a gene sequences. The neighbour‐joining method of the mega program was used with bootstrap resampling (1000 replicates) to place approximate confidence limits on individual branches; only bootstrap values above 80 are shown. GenBank numbers: n–n–n–n–n–n–n–n. CAN, Canada; Q, Australia; NL, the Netherlands; BE, Belgium; KR, Korea. NL63‐Amst1 is the prototype sequence of HCoV‐NL63. Red and blue indicate the strains that cluster for the 1a region and for which spike sequences are also available.