Fig. 3.
Characterization of the p64/p12 complex. After purification with the glutathione Sepharose 4B column, the full-length GST-RdRp protein sample was stored at 4 °C for 5 days. One portion was treated with thrombin for the cleavage of GST and another was not treated with thrombin. Both samples were further purified with the polyA Sepharose 4B column. (a) SDS-PAGE of the purified protein samples without (left panel) and with thrombin treatment (right panel). (b) Native PAGE of GST (left panel) and the purified protein sample with thrombin treatment (right panel). (c) IEF gel of GST (left panel) and the purified protein sample with thrombin treatment (right panel).