Table 2.
Concentration and detection of SARS-CoV from 2500-ml sewage in the 309th Hospital of PLAa
| Date | Patients in hospital | Patientsb with symptoms | f2 input | f2 recovered (%) | Cell culturec | Concentrate + PCRd | Inoculated cell + PCRe | Residual chlorinea |
|---|---|---|---|---|---|---|---|---|
| 11 June | 7 | 0 | 1.3 × 106 | 93.1 | −f | +g | + | − |
| 12 June | 6 | 0 | 2.1 × 106 | 87.5 | − | + | + | − |
| 13 June | 3 | 0 | 1.3 × 106 | 96.9 | − | + | + | − |
| 14 June | 2 | 0 | 1.3 × 106 | 70.0 | − | + | + | − |
| 15 June | 2 | 0 | 3.0 × 105 | 62.2 | − | + | + | − |
a
Glass column diameter: 19 mm, bed height: 14 cm, eluate volume: 500 ml.
b
With any one of the symptoms of fever, malaise, cough, dyspnea, except chest radiography signs.
c
Cell culture was maintained for 14 days to observe the cytopathic effect.
d
PCR template was from the concentrates.
e
Template from the cultured cells.
f
Negative.
g
Positive.