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. 2012 Feb 10;165(1):112–117. doi: 10.1016/j.virusres.2012.02.002

Table 1.

Particle set Target shRNA sequence (5′ → 3′)
(A) shRNA sequences used for lentiviral-based gene knockdown
Non-target control
TRC1.5 Vector (pLKO.1-puro)		 CCGGCAACAAGATGAAGAGCACCAACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTTG
PPIB: SHVRS-NM_000942 TRCN000049251 CCGGGTTCTTCATCACGACAGTCAACTCGAGTTGACTGTCGTGATGAAGAACTTTTTG
FKBP1A: SHVRS-NM_054014 TRCN0000005951 CCGGGCCAAACTGACTATATCTCCACTCGAGTGGAGATATAGTCAGTTTGGCTTTTT
FKBP1B: SHVRS-NM_004116 TRCN00000151663 CCGGGAAGTTTGATTCATCCAGAGACTCGAGTCTCTGGATGAATCAAACTTCTTTTTTG
qRT-PCR primer Sequence (5′ → 3′)
(B) Sequences of primers used for quantification of gene knockdown
hPPIB_F CTCTCCGAACGCAACATGAAG
hPPIB_R ACCTTGACGGTGACTTTGGG
h_FKBP1A_F CACTACACCGGGATGCTTGAA
h_FKBP1A_R TTCTTCCCAGCCTCGGATCA
h_FKBPB_F GCAGGAAGGAACTCAAGGTG
h_FKBP1B_R AGCAACTTGGGCAGAGAGAA