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Gating strategy. Representative flow cytometry plots from healthy controls (HC), patients with decompensated liver cirrhosis (DLC), and patients with SBP. MAIT cells were identified as viable CD3+ lymphocytes simultaneously expressing CD161 at high levels (CD161hi) and the invariant TCR Vα7.2. Representative tetramer staining of MR1 loaded with the pyrimidine intermediate 5-OP-RU is shown (bottom right panel). FSC, forward scatter; SSC, side scatter.
