. 2020 May 23;736:139652. doi: 10.1016/j.scitotenv.2020.139652

Table 1.

Primers and amplification protocols used in the study.

Target	Region	Primer name	Nucleotide sequence	Orientation	Usage	Amplicon size (bp)	Reference
Broad-range coronavirus	ORF1ab	Bat-CoV pol 15197	GGTTGGGAYTAYCCWAARTGTGA	+	First PCR	440	Ar Gouilh et al. (2018)
		Bat-CoV pol 15635	CCATCRTCMGAHARAATCATCATA	−	First PCR	440
		Bat-CoV pol 15419	GTGCTAAACCACCGCCTG	+	Nested PCR	218
		Bat-CoV pol 15635	CCATCRTCMGAHARAATCATCATA	−	Nested PCR	218
SARS-CoV-2	ORF1ab	2274 - CO-FW1	GTGCTAAACCACCGCCTG	+	First PCR	368	This study
		2275 - CO-REV1	CAGATCATGGTTGCTTTGTAGGT	−	First PCR	368
		2276 - CO-FW2	CGCCTGGAGATCAATTTAAACAC	+	Nested PCR	332
		2277 - CO-REV2	ACCTGTAAAACCCCATTGTTGA	−	Nested PCR	332
SARS-CoV-2	S	WuhanCoV-spk1-f	TTGGCAAAATTCAAGACTCACTTT	+	First PCR	547	Nao et al. (2020)
		WuhanCoV-spk2-r	TGTGGTTCATAAAAATTCCTTTGTG	−	First PCR	547
		NIID_WH-1_F24381	TCAAGACTCACTTTCTTCCAC	+	Nested PCR	493
		NIID_WH-1_R24873	ATTTGAAACAAAGACACCTTCAC	−	Nested PCR	493
SARS-CoV-2	RdRP	RdRP_SARSr-F2	GTGARATGGTCATGTGTGGCGG	+	Real-time RT-qPCR	−	Corman et al. (2020)
		RdRP_SARSr-R1	CARATGTTAAASACACTATTAGCATA	−
		RdRP_SARSr-P2	FAM-CAGGTGGAACCTCATCAGGAGATGC- BHQ1