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. Author manuscript; available in PMC: 2020 Nov 15.
Published in final edited form as: Sci Immunol. 2020 May 15;5(47):eaaz6894. doi: 10.1126/sciimmunol.aaz6894

Fig. 3. Single-cell RNA sequencing analyses highlight heterogeneity among circulating CD8+ T cells.

Fig. 3.

(A) tSNE analysis of splenic CD8+ T cells; each plot represents an individual time point with each color representing a specific time point, as in Fig. 1B. (B) Relative expression (compared to mean expression among all spleen cells) of known regulators of circulating CD8+ T cell differentiation superimposed on individual spleen cells. (C and D) Similarity of gene-expression programs among single spleen cells to transcriptional signatures (derived from bulk RNA-seq profiles from FACS-sorted cells) of previously defined circulating CD8+ T cell subsets. (C) Similarity of gene-expression programs among single spleen cells to terminal effector (TE) (D7 KLRG1hiIL-7Rαlo, red) or memory-precursor (MP) (D7 KLRG1loIL-7Rαhi, blue) transcriptional signatures. (D) Similarity of gene-expression programs among single spleen cells to LLE (D35 CD62LloIL-7Rαlo, red), TEM (D35 CD62LloIL-7Rαhi, blue), and TCM (D35 CD62LhiIL-7Rαhi, green) cell transcriptional signatures.