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. 2020 Dec 1;33(12):108528. doi: 10.1016/j.celrep.2020.108528
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
anti-HA antibody Covance Cat# MMS-101R; RRID:AB_291262
anti-His Invitrogen Cat# 372900; RRID:AB_2533309
anti-GAPDH Life Technologies Cat# AM4300; RRID:AB_2536381
anti-p24 antibody (AG3.0) AIDS respository Cat# 4121; RRID:AB_2734137
anti-ACE2 antibody (AC384) NOVUS Cat# NBP2-80038
Anti-spike antibody GeneTex Cat# GTX632604; RRID:AB_2864418
Alexa Fluor 594-conjugated goat anti-mouse IgG Biolegend Cat# 405326; RRID:AB_2563308
goat anti-mouse HRP-conjugated second antibody Sigma Cat# A4416; RRID:AB_258167
Bacterial and Virus Strains
VSV-G pseudotyped reporter virus This paper N/A
SARS-CoV-2 full-length S pseudotyped reporter virus This paper N/A
SARS-CoV-2 Δ19 S pseudotyped reporter virus This paper N/A
SARS-CoV-2 Δ19 S D614G pseudotyped reporter virus This paper N/A
SARS-CoV pseudotyped reporter virus This paper N/A
WIV1 pseudotyped reporter virus This paper N/A
LYRa11 pseudotyped reporter virus This paper N/A
Rs4231 pseudotyped reporter virus This paper N/A
Rs4084 pseudotyped reporter virus This paper N/A
SHC014 pseudotyped reporter virus This paper N/A
mNeonGreen SARS-CoV-2 Xie et al. (2020), Cell Host and Microbe N/A
SARS-CoV-2 isolate USA-WA1/2020 BEI Resources Cat# NR-52281
Biological Samples
convalescent sera NYU Vaccine Center with written consent under I.R.B. approval IRB 20-00595 and IRB 18-02037
Chemicals, Peptides, and Recombinant Proteins
nevirapine NYU Pharmacy Cat# 4666
ACE2 microbody This paper N/A
ACE2.H345A microbody This paper N/A
Soluble ACE2 This paper N/A
polyethyleneimine Polysciences Cat# 23966-1
Critical Commercial Assays
GIBCO ExpiFectamine CHO Transfection Kits Thermo Fisher Scientific Cat# A29130
Nano-Glo® Luciferase Assay System Promega Cat# N1120
Nickel-nitrilotriacetic acid-agarose beads QIAGEN Cat# D-40724
Dawn Heleos II 18-angle light-scattering detector Wyatt Technology https://www.wyatt.com/products/instruments/dawn-multi-angle-light-scattering-detector.html
Dynamic light-scattering detector Wyatt Technology https://www.wyatt.com/products/instruments/dynamic-electrophoretic-light-scattering-detectors.html
Optilab t-rEX refractive index detector Wyatt Technology Cat# Optilab T-rEX
Experimental Models: Cell Lines
293T ATCC N/A
ACE2.293T This paper N/A
Vero ATCC N/A
Vero E6 ATCC N/A
CaCO2 ATCC N/A
A549 Benjamin tenOever (ISMMS) N/A
ACE2.A549 This paper N/A
BHK ATCC N/A
Huh7 ATCC N/A
CHME3 ATCC N/A
U937 ATCC N/A
CHO Thermo Fisher Cat# A29130
293F Thermo Fisher Cat# R79007
Experimental Models: Organisms/Strains
K18-hACE2 mice [B6.Cg-Tg(K18-ACE2)2Prlmn/J Jackson Laboratory Stock No: 034860
Oligonucleotides (5”-3”)
See Table S1 for primers IDT This paper
Recombinant DNA
S fragment A (cDNA). See Table S2 for sequence Thermo Fisher This paper
S fragment B (cDNA). See Table S2 for sequence Thermo Fisher This paper
ACE2 (cDNA). See Table S2 for sequence Origene This paper
pLenti.CMV.GFP.puro Addgene Cat# 17448
Software and Algorithms
MacVector ver 17.0.0 (27) MacVector Inc N/A
GraphPad Prism 7 7.0e Software GraphPad Prism Software, Inc. N/A
FlowJo 10.5.3 FlowJo, LLC N/A
ASTRA 6 software Wyatt Technology N/A