Table 1.
Sensitivity and specificity of ELISA and LFRET in detection of SARS-CoV-2 -PCR-positive individuals for all patients and hospitalized patients at different time points from symptom onset. Absolute numbers of samples are stated after the percentages. Altogether, 19 samples from nine PCR-positive individuals for whom the onset of symptoms is unknown are excluded. NP = nucleoprotein. SP = spike glycoprotein. LFRET = protein L–based time-resolved Förster resonance energy transfer immunoassay. ELISA = enzyme-linked immunosorbent assay.
| Patient Group | All Samples | >8 Days after Onset |
>13 Days after Onset |
All Samples | >8 Days after Onset |
>13 Days after Onset |
|---|---|---|---|---|---|---|
| SP LFRET sensitivity (specificity 100% (52/52)) | SP IgG ELISA sensitivity (specificity 100% (52/52)) | |||||
| All patients | 86% (50/58) | 92% (49/54) | 95% (38/40) | 90% (52/58) | 94% (51/54) | 100% (40/40) |
| Hospitalized | 83% (24/29) | 92% (23/25) | 100% (13/13) | 83% (23/29) | 92% (23/25) | 100% (13/13) |
| NP LFRET sensitivity (specificity 100% (52/52)) | NP IgG ELISA sensitivity (specificity 94% (49/52)) | |||||
| All patients | 76% (44/58) | 81% (44/54) | 80% (32/40) | 93% (54/58) | 98% (53/54) | 100% (40/40) |
| Hospitalized | 79% (23/29) | 92% (23/25) | 100% (13/13) | 86% (25/29) | 96% (24/25) | 100% (13/13) |
| SP/NP LFRET sensitivity (specificity 100% (52/52)) | SP/NP IgG ELISA sensitivity (specificity 94% (49/52)) | |||||
| All patients | 90% (52/58) | 94% (51/54) | 95% (38/40) | 93% (54/58) | 98% (53/54) | 100% (40/40) |
| Hospitalized | 86% (25/29) | 96% (24/25) | 100% (13/13) | 86% (25/29) | 96% (24/25) | 100% (13/13) |