Table 3.
Tau modifications quantified in hAPP and wildtype mice.
| Modification site(s) | PTM | No. of modified residues | Modified residue (mouse 430 isoform) | Homologous human residue (441 isoform) | Quantified in whole lysate | Quantified in PSD |
|---|---|---|---|---|---|---|
| DHGLKESPP | GlyGly | 1 | 33a | 44 | + | – |
| DHGLKAEEA | GlyGly | 1 | 33b | 44 | + | – |
| PGSETSDAK | Phospho | 1 | 52a | 63 | + | – |
| SDAKSTPTAE | Phospho | 1 | 57|58a | 68|69 | + | – |
| GIGDTPNQE | Phospho | 1 | 100c | 111 | + | – |
| IATPRGAAS | Methyl | 1 | 144 | 155 | + | – |
| RGAASPAQKGTSNATRIPA | Phospho | 2 | 148, 158d | –e, 169 | – | + |
| IPAKTTPSPKTPPGSGEPPKSGERS | Phospho | 1 | 164|170|174|180 | 175|181|185|191 | + | + |
| IPAKTTPSPKTPPGS | Phospho | 2 | 164|167|170 | 175|–e| 181 | + | – |
| IPAKTTPSPKTPPGS | Phospho | 3 | 164, 167, 170 | 175, –e, 181 | + | – |
| PSPKTPPGSGEPPKSGERS | Phospho | 2 | 170, 180 | 181, 191 | – | + |
| SGERSGYSSPGSPGTPGSR | Phospho | 1 | 184|187|191|194 | 195|198|202|205 | + | + |
| SGERSGYSSPGSPGTPGSR | Phospho | 2 | 184|187|188|191|194 | 195|198|199|202|205 | + | + |
| RSGYSSPGSPGTPGSRSRTPS | Phospho | 3 | 187|188|191|194|199 | 198|199|202|205|210 | + | + |
| PGSRSRTPSLPTPPTR | Phospho | 1 | 199|201|203|206 | 210|212|214|217 | + | + |
| PGSRSRTPSLPTPPTR | Phospho | 2 | 199|201|203|206 | 210|212|214|217 | + | – |
| AVVRTPPKSPSASKS | Phospho | 1 | 220|224|226 | 231|235|237 | + | + |
| AVVRTPPKSPSASKSRL | Phospho | 2 | 220|224|226|228 | 231|235|237|239 | + | – |
| NVRSKIGST | Acetyl | 1 | 248 | 259 | + | – |
| SKIGSTENL | Phospho | 1 | 251 | 262 | + | – |
| IINKKLDLS | Acetyl | 1 | 270 | 281 | + | + |
| IINKKLDLS | GlyGly | 1 | 270 | 281 | + | – |
| VKSEKLDFK | Acetyl | 1 | 332 | 343 | + | – |
| KLDFKDRVQ | Acetyl | 1 | 336 | 347 | + | – |
| KLDFKDRVQ | GlyGly | 1 | 336 | 347 | + | – |
| SKIGSLDNI | Phospho | 1 | 345 | 356 | + | + |
| IETHKLTFR | GlyGly | 1 | 364 | 375 | + | – |
| NAKAKTDHG | Acetyl | 1 | 374 | 385 | + | – |
| AKAKTDHGAEIVYKSPVVSGDTSPRHL | Phospho | 2 | 375|385|389|392|393 | 386|396|400|403|404 | + | + |
| AKAKTDHGAEIVYKSPVVSGDTSPRHL | Phospho | 3 | 375|385|389|392|393 | 386|396|400|403|404 | + | + |
| IVYKSPVVS | Phospho | 1 | 385 | 396 | + | + |
| SPVVSGDTSPRHL | Phospho | 1 | 389|392|393 | 400|403|404 | + | + |
| PRHLSNVSSTGSIDMV | Phospho | 2 | 398|401|402|405 | 409|412|413|416 | + | + |
| SSTGSIDMV | Phospho | 1 | 405f | 416 | + | + |
Sites for which unambiguous assignment was not possible are indicated with a vertical bar between alternative assignments.
a
Peptide from mouse isoform A.
b
Peptide from mouse isoforms B, C, or D.
c
Peptide from mouse isoforms A, B, C, or D.
d
Identified in PSD experiments.
e
Not conserved in human tau.
f
Site unambiguously assigned with AspN digestion.