Figure 3.
Detection of β‐amyloid precursor protein (β‐APP)‐positive axons (A,B) in cerebellar white matter distemper lesions without (C,E) or with demyelination (D,F). A. Area of antigen detection without visible lesion (group 3) with single β‐APP‐positive axons in the cerebellar white matter [arrows; brown: β‐APP, avidin‐biotin‐peroxidase complex (ABC) method, 3,3′‐diaminobenzidine tetrahydrochloride (DAB)]. B. Subacute non‐inflammatory lesion (group 6) with numerous β‐APP‐positive axons in the cerebellar white matter (arrows; brown: β‐APP, ABC method, DAB). C. Serial section of A. Lesion of group 3 with complete myelination [asterisk; luxol fast blue (LFB) stain]. D. Serial section of B. Subacute non‐inflammatory lesion (group 6) with moderate to severe demyelination in the cerebellar white matter (asterisk; LFB stain). E. Serial section of A and C. Lesion of group 3 with single, black‐colored, β‐APP‐positive axons (arrows) within an area of complete myelination (asterisk; black: β‐APP, ABC method, DAB in combination with LFB stain). F. Serial section of B and D. Subacute non‐inflammatory lesion (group 6) with numerous, black‐colored, β‐APP‐positive axons (arrows) in an area with moderate to severe demyelination (asterisk; black: β‐APP, ABC method, DAB in combination with LFB stain). A,B, E,F. Bar = 25 µm. C,D. Bar = 50 µm.