FIG. 3.
Programmed −1 ribosomal frameshifting (−1 RFS) in a mak8-1 strain is not further affected by peptidyltransferase inhibitors. Isogenic wild-type and mak8-1 cells harboring either p0 or p−1 frameshift indicator plasmids were grown in the presence of the indicated concentrations of sparsomycin (A) or anisomycin (B) for 4 h, after which programmed −1 ribosomal frameshifting efficiencies were determined as described in Materials and Methods. In the absence of drugs, wild-type cells promote approximately 2% efficiency of programmed −1 ribosomal frameshifting, whereas this value is approximately 5% in cells harboring the mak8-1 allele. The fold changes in programmed −1 ribosomal frameshifting efficiencies are plotted on the y axis.