TABLE 2.
Assays of programmed −1 ribosomal frameshifting and the killer phenotype in yeast cells harboring the wild-type RPL3 gene or the mak8-1 allele
| Strain | % Ribosomal frameshifta
|
Killer phenotypeb | |
|---|---|---|---|
| −1 | +1 | ||
| 1906 (mak8-1) | 5.18 ± 0.12 | 4.12 ± 0.16 | − |
| 980-10C pRPL3 | 1.93 ± 0.18 | 5.34 ± 0.18 | + |
| 980-10C + pmak8-1 | 4.85 ± 0.12 | 5.47 ± 0.11 | − |
a
The percent −1 ribosomal frameshifting was calculated by multiplying the ratio of p−1/p0 β-galactosidase activities by 100%. Absolute error is shown.
b
Killer phenotype was determined as described in Materials and Methods.