TABLE 1.
Identification of the nuclear import signal of hTAPa
| hTAP aa | GFP fusion
|
GFP–β-Gal fusion
|
||
|---|---|---|---|---|
| Nuclear accumulation | Nuclear rim association | Local-ization | Nuclear rim association | |
| 1–619 | Yes | Yes | N | Yes |
| 1–60 | No | No | C | No |
| 61–619 | Yes | Yes | N | Yes |
| 61–560 | Yes | No | ||
| 61–507 | Yes | No | ||
| 61–380 | Yes | No | N | No |
| 61–341 | Yes | No | ||
| 61–273 | Yes | No | ||
| 61–175 | Yes | No | ||
| 61–160 | Yes | No | ||
| 61–140 | Yes | No | ||
| 61–134 | Yes | No | ||
| 61–128 | Yes | No | N | No |
| 61–120 | Yes | No | N | No |
| 61–110 | N | No | ||
| 61–100 | Yes, impaired | No | N+C | No |
| 61–80 | No | No | C | No |
| 67–140 | Yes | No | ||
| 81–140 | No | No | C | No |
| 95–140 | No | No | ||
| 1–619 R/A 69–82 | Yes, impaired | Yes | C | Yes |
| 61–140 (Δ81–109) | Yes, impaired | No | C | No |
| 61–140 (Δ81–119) | Yes, impaired | No | C | No |
| 61–120 R/A 97–105 | N+C | No | ||
| 61–140 R/A 69–82 | No | No | C | No |
| 61–140 R/A 78,81 | C | No | ||
a
N, nucleus; C, cytoplasm.