TABLE 2.
Oligonucleotides used
| Oligonucleotide | Sequence (5′–3′) |
|---|---|
| UME6-NcoI | CCTTAACTCACCATGGTAGACAAGGCGCG |
| UME6-Asp718 | GCGGTGGTACCGGGGGAAG |
| UME6-T99A | AACAACGCTCCCGTGCACACTCCGTCTGGTTCG |
| UME6-T99A-3′ | GTGCACGGGAGCGTTGTTGGGCGCACATGTCGA |
| UME6-T103A | GTGCACGCTCCGTCTGGTTCGCCGAGTTTG |
| UME6-T103A-3′ | AGACGGAGCGTGCACGGGAGTGTTGTTGGGCGCACA |
| UME6-S107A | GTGCACACTCCGTCTGGTGCACCGAGTTTGAAAGTCC |
| UME6-S107A | CGGTGCACCAGACGGAGTGTGCACGGGAGTGTTGTTGGGCGCACATGTCGA |
| UME6-Ala3 | AACGCTCCCGTGCACGCTCCGTCTGGTGCACCGAGTTTG |
| UME6-Ala3-3′ | CGGTGCACCAGACGGAGCGTGCACGGGAGCGTTGTTGGG |
| UME6-up | GAAGCGCCCACCTTCGCACAGCGCACAGGAACTAGGACACTACCGCACTCAAACCATTTGGCAGATTGTACTGAGAGTGC |
| UME6-dn | GATTTCCTCCAGTTTCATCTGTTTTTTCTTTGGATCAGATACAAAATCTGGTTTGAACGCCGCATCTGTGCGGTATTTCAC |
| MRK1.-60+kl.URA3 | GGGGAAGGGACTTGAAAATAATCTCAAGATTGAAGAGGTGAAAATTGTAAAATAGTCTACTCATGGCAATTCCCGGGGATC |
| MRK1.1860.3′+kl.URA3 | CAAATGCATATTATGTAATAGTAATGATACAATAGACTAAGAAATTTGAAGGATGAGATATAATGGTGGTCAGCTGGAATTC |
| UME6.-100 | GAGACCAGTGATCGTAAG |
| pRS-marker-3′ | CGCATCTGTGCGGTATTTC |
| SmaI+MCK1.1 | CCCGGGAATGTCTACGGAAGAGCAGAA |
| MCK1.1628-3′ | TTATTCAGCAACTTTCGTAG |
| NotI-kl.URA3-1321-3′ | AGCGGCCGCATGGTGGTCAGCTGGAAT T |
| MRK1.-104 | AGAACCCTGCTGCATACAAG |