. 2001 Aug;39(8):2768–2778. doi: 10.1128/JCM.39.8.2768-2778.2001

TABLE 3.

Oligonucleotide primers used for ITS amplification and sequencing

Primer	Bartonella species	Nucleotide sequence (5′ to 3′)	Reference(s)
16SF^a ^b	All (except B. bacilliformis)	AGAGGCAGGCAACCACGGTA	47, 48
23S1^a ^b ^c	All (except B. bacilliformis)	GCCAAGGCATCCACC	47, 48
QHVE1^b	All	TTCAGATGATGATCCCAA	47, 48
QHVE2^b ^c	All	TTGGGATCATCATCTGAA	47, 48
QHVE3^b	All	GATATATTCAGACATGTT	47, 48
QHVE4^b ^c	All	AACATGTCTGAATATATC	47, 48
BABF^a ^b	B. bacilliformis	CTGGATCACCTCCTTTCTAA	This study
BABR^a ^b ^c	B. bacilliformis	ATGCCCTTAAGACACTTGAT	This study
BQF^a ^b	B. quintana	CTCCACCATTTTAGGTCATC	This study
BQR^a ^b ^c	B. quintana	GGTTTTGAGAATTCCCTTGC	This study

Primer used for PCR. The 16SF primer was chosen from a conserved region of the 3′ end of the 16S rRNA-encoding gene of Bartonella species, and the 23S1 primer was chosen from the antisense strand of the 23S rRNA-encoding gene.

Primer used to sequence the PCR amplification product.

The primer was located on the complementary strand of DNA at the same position as the primer preceding it in the table.