TABLE 1.
B. burgdorferi chromosome copy number
| Sample | Quantification method | na | Ratiob |
|---|---|---|---|
| Purified B. burgdorferi | Limiting-dilution assay | 24 | 1.3 ± 0.41 |
| recA PCR product as external standard | 3 | 0.22 ± 0.030 | |
| Limiting dilution assay in presence of 200 ng of joint DNA | 24 | 0.92 ± 0.26 | |
| Culture-spiked spleen | B. burgdorferi DNA as external standard in presence of 200 ng of spleen DNA | 2 | 1.0 ± 0.12 |
a
n, number of samples.
b
Ratio, number of recA molecules per estimated number of bacteria in sample. The error values are standard deviations of replicate samples.