TABLE 1.
PCR amplification of aminoglycoside resistance genes
| Gene(s) | Primer pair (5′→3′)a | Product size (bp) | Refer-ence |
|---|---|---|---|
| aadE | (1268) ACT GGC TTA ATC AAT TTG GG | 597 | 13 |
| (1845) GCC TTT CCG CCA CCT CAC CG | |||
| aadA | (564)b TGA TTT GCT GGT TAC GGT GAC | 284 | 16 |
| (829)b CGC TAT GTT CTC TTG CTT TTG | |||
| aac(6′)-aph(2") | (348) TGA TGA TTT TCC TTT GAT GT | 1,395 | 14 |
| (1723) CAA TCT TTA TAA GTC CTT TT |
a
The numbers in parentheses denote the positions of the initial nucleotides in the primer sequences.
b
Since the aadA gene sequences for several different organisms are published, the nucleotide number varies and the primers were selected from within a universally conserved sequence.