. 2000 Sep;66(9):3664–3673. doi: 10.1128/aem.66.9.3664-3673.2000

TABLE 1.

16S rRNA-targeted oligonucleotide probes and PCR primers used in this study

Short name	Target taxon(a)	Sequence (5′-3′)	Position^a	OPD full name^b	Wash temp (°C)^c	Reference
Univ1390	Universal	GAC GGG CGG TGT GTA CAA	1407–1390	S-^*-Univ-1390-a-A-18	44	55
Entero	Enterobacteria	CTT TTG CAR CCC ACT	1432–1418	not available	44	31
Lacb0722	All LAB	YCA CCG CTA CAC ATG RAG TTC CAC T	746–722	S-^*-Lab-0722-a-A-25	54	42
Strc493	Streptococcus, Lactococcus, some Leuconostoc	GTT AGC CGT CCC TTT CTG G	511–493	S-^*-Strc-0493-a-A-19	50	16
212RLa	Lactococcus	CTT TGA GTG ATG CAA TTG CAT C	233–212	S-S-L.lac-0212-a-A-22	46	38
Lab158	Lactobacillus, Enterococcus, Pediococcus, Leuconostoc, Weissella	GGT ATT AGC AYC TGT TTC CA	177–158	S-G-Lab-0158-a-A-20	45	7
LU2	Leuconostoc	GAT CCA TCT CTA GGT GAC GCC G	242–220	not available	46	32
Lbfe	L. fermentum	GCG ACC AAA ATC AAT CAG G	90–72	S-S-Lb.ferm-0072-a-A-19	50	40
Lbpe	L. plantarum	TCA AAT GTA AAT CAT GAT G	90–72	S-S-Lb.plan-0072-a-A-19	38	40
lm3	Bifidobacterium	CGG GTG CTI CCC ACT TTC ATG	1432–1412	S-G-Bif-1412-a-A-21	49.5	24
Euk1427f^d	NA^e	TCT GTG ATG CCC TTA GAT GTT CTG GG	1453–1427	NA	NA	48
Euk1616r	NA	GCG GTG TGT ACA AAG GGC AGG G	1616–1637	NA	NA	48
338f^d	NA	ACT CCT ACG GGA GGC AGC AG	357–338	NA	NA	25
518r	NA	ATT ACC GCG GCT GCT GG	534–518	NA	NA	30

E. coli numbering, except for Euk1427f and Euk1616r (Saccharomyces cerevisiae numbering).

OPD, Oligonucleotide Probe Database (4).

Wash temperature in 1× SSC–1% sodium dodecyl sulfate (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate).

A GC clamp was attached to the 5′ ends of the 338f and Euk1427f primers to obtain primers gc338f and gcEuk1427f (GC clamp: 5′CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGG) (30).

NA, not applicable.