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. 2001 Jun;67(6):2760–2765. doi: 10.1128/AEM.67.6.2760-2765.2001

TABLE 3.

Direct Multiplex-PCR analysis of 18 commercially available product samples with declared content of bifidobacteria (samples 1 to 4 and 6 to 18), lactobacilli (sample 5), or other LABb

Sample no. PCR results
Bflact2-Bflact5 Lm3-P0a P0-338F
1 + ± +
2 + ± +
3 − + +
4 − + +
5 − − +
6 + ± +
7 + ± +
8 − + +
9 − + +
10 + ± +
11 + ± +
12 + ± +
13 + ± +
14 + ± +
15 + ± +
16 − + +
17 − + +
18 − + +
a

±, PCR results after a run of 25 cycles. For B. lactis, a competitive reaction between the resulting PCR amplicons for the species B. lactis and the genus Bifidobacterium might occur (primer pair Bflact2-Lm3). 

b

LAB, lactic acid bacteria.