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Synthesis of NAD from NAm and PRPP by extracts of A. pleuropneumoniae containing nadVa
| Time (min) | NAm (μmol) | NMN (μmol) | NAD (μmol) |
|---|---|---|---|
| 0 | 360 | 25 | 25 |
| 30 | 220 | 40 | 123 |
Reaction mixture contained cell extract, 80 mM potassium phosphate buffer (pH 7.4), 16 mM MgCl2, 1 mM ATP, 5mM PRPP, and 2 mM NAm, incubated for 30 min at 37°C. Data are from a representative experiment. Trends were identical in all experiments.
