TABLE 1.
Effect of a broad-spectrum caspase inhibitor on C. trachomatis-induced PS externalization
| PS agonist | Mean fluorescencea
|
||
|---|---|---|---|
| Medium | z-VAD-fmkb | % Decreasec | |
| None | 2.25 | 2.22 | 1.3 |
| 5% Ethanold | 3.01 | 1.92 | 36.2 |
| C. trachomatis | 5.10 | 4.59 | 10 |
a
Mean fluorescence data of 50,000 gated events gathered by flow cytometry.
b
THP-1 cells were pretreated with 100 μM z-VAD-fmk or dimethyl sulfoxide (medium) for 1 h at 37°C before addition of the PS agonist.
c
Percent decrease = [(medium fluorescence) − (z-VAD-fmk fluorescence)]/(medium fluorescence) × 100.
d
Cells were incubated with 5% ethanol or 1 IFU of C. trachomatis/ml for 1 h at 37°C in the presence or absence of z-VAD-fmk. Uninfected cells were cultured in unsupplemented IMDM.