TABLE 1.
Characteristics of pneumococci grown under various conditions.
| Characteristic | Colony morphologya of:
|
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|---|---|---|---|---|---|---|
| O variant under (%O2, %CO2):
|
T variant under (%O2, %CO2):
|
|||||
| 21, <0.1 | 16, 3 | <0.1, 10 | 21, <0.1 | 16, 3 | <0.1, 10 | |
| Avg colony area (mm2)b | 3.5 | 7.4 | 170 | 1.0 | 5.2 | 12 |
| Vol occupied by a single cell with capsule zone (μm3)c | 3.6 | 6.8 | 63 | ND | 4.6 | 11 |
| CPS (ng/μg of total protein)d | 130 | 220 | 990 | 35 | 19 | 34 |
For O and T variants of the same type 6A clinical isolate, P303. ND, zone of capsular material was undetectable.
Based on the colony diameter after 16 h of growth at 37°C on nutrient agar. Values represent the average of determinations on five single-well isolated colonies.
Based on the size of the refractile zone visualized with the quellung reaction and the formula for the volume of a spherical ellipsoid: V = (π/6)LW2. The values represent the average of determinations for three diplococcal forms divided by two to provide an estimate for a single cell.
Based on a capture ELISA of organisms grown in liquid culture to mid-log phase. The values are the mean of two separate determinations performed in duplicate.