TABLE 15.
A sample of archaeal hsp60 (chaperonin) genesa
| Organism | Chaperonin (accession no.) | Size (bp) | Promoter |
|---|---|---|---|
| Archaeoglobus fulgidus | thsA/Cpn-α (AE000950) | 1,635 | tatata (−49)b, tgcaa (−19) |
| thsB/Cpn-β (AF035826) | 1,635 | cttata (−49), tgcaa (−19) | |
| Desulforococcus strain SY | HHSP (S79557) | 1,635 | aggagg (−15) |
| Haloferax volcanii | cct1 (AF010470) | 1,683 | tttata (−26), cgaa (−34) |
| cct2 (AF010469) | 1,674 | tttata (−26), cgaa (−34) | |
| cct3 (AF029873) | Partial seq. | tttata (−26), cgaa (−36) | |
| Methanobacterium thermoautotrophicum ΔH | Chaperonin (α-subunit) (MT0794) | 1,617 | NR |
| Chaperonin (MT0218) | 1,659 | NR | |
| Methanococcus jannaschii | Chaperonin (U67542) | 1,626 | NR |
| Methanococcus thermolithotrophicus | MTTS (AB015435) | 1,632 | tttatata (−75) |
| Methanopyrus kandleri | Thermosome (Z50745) | 1,635 | tttaaata (−60), atgc (−42) |
| Pyrobaculum aerophilum | TF55 (PA306e) | NR | NR |
| TF56 (PA307e) | NR | NR | |
| TF55 (PA27e) | NR | NR | |
| Pyrococcus kodakaraensis KOD1 | cpkA (AB018432) | 1,647 | NR |
| cpkB (D29672) | 1,641 | NR | |
| Pyrococcus abyssi | α-Subunit-thsA (PAB2410) | 1,650 | NR |
| Sulfolobus shibatae | TF55-β (X63834) | 1,659 | tttata (−40) |
| TF56-α (L34691) | 1,680 | tttata (−40) | |
| Sulfolobus sp. strain 7 | α-Subunit (AB001085) | 1,680 | ttttatata (−37) and tgc (−18) |
| β-Subunit (AB001086) | 1,659 | ttttatata (−37) and tgc (−18) | |
| Thermococcus strain KS-1 | α-Subunit (AB001080) | 1,638 | NR |
| β-Subunit (AB001081) | 1,647 | NR | |
| Thermoplasma acidophilum | α-Subunit (Z46649) | 1,638 | tttata (−40) |
| β-Subunit (Z46650) | 1,632 | ttatata (−33), tttatttttta (−21) | |
| Aeropyrum pernix K1 | Thermosome subunit (AEP0907) | 1,671 | NR |
| Thermosome subunit (AEP2072) | 1,665 | NR |
| Terminator | RBS | Remarks | Reference(s) |
|---|---|---|---|
| NRc | aggtg (−9) | Enhanced Box A elementd | 74, 151 |
| NR | aggtg (−9) | Enhanced Box A element | |
| tttga–t-rich-9–tcaaa (+18) | NR | 140 | |
| NR | NR | Induced by heat and hypotonic shocks; enhanced Box A element | 163, 277 |
| NR | NR | Induced by heat and hypotonic shocks; enhanced Box A element | |
| NR | NR | Induced by heat shock | |
| NR | NR | 263 | |
| NR | NR | ||
| NR | NR | 31 | |
| t-rich region (+20) | NR | Recombinant | 84 |
| c-rich region | aggtgat (+18) | 3 | |
| NR | NR | 79 | |
| NR | NR | ||
| NR | NR | ||
| NR | NR | 137, 305 | |
| NR | NR | ||
| NR | NR | P. abyssi website | |
| ttttttt (+7) | aggtg (−8) | Enhanced Box A element | 141, 281 |
| ttttttt (+6) | tgaggt (−4), ggtg (−12) | Enhanced Box A element | |
| NR | ggtg (−6) | 207 | |
| NR | ggtg (−9) | ||
| NR | NR | Recombinant | 308 |
| NR | NR | Recombinant | |
| t-rich region (+6 to +38) | atgcg (−22), aggtgat (−10), caggttcc (+16), tccat (−12) | 288 | |
| ttttt and aaaaa (+14), attta-9-taaat (+35) | tccat (−12) | ||
| NR | NR | 143 | |
| NR | NR |
b
(−) and (+) refer to position of center of sequence upstream from translation start codon or downstream from translation stop codon, respectively.
c
NR, not reported.
d
Conserved sequences as an extension of Box A and between Box A and Box B.
e
Identification number used in genome project.