TABLE 7.
Archaeal Hsp40(DnaJ) amino acid sequences deduced from cloned genes: motifsa
| Motif | Function | Reference(s) | Synonym | Reference(s) | Motif presentc
|
|||
|---|---|---|---|---|---|---|---|---|
| M. mazei S-6 (181) | M. thermophila TM-1 (126) | M. thermoautotrophicum ΔH | M. cutirubrum (32) | |||||
| J-domain | Regulates Hsp70 ATPase; acts as competitive inhibitor of DnaJ in protein refolding | 176, 272, 284 | N terminus | 37 | Yes | Yes | Yes | Yes |
| G-rich domain | Flexible spacer between domains; aids J-domain in stimulating Hsp70 ATPase; competitive inhibitor of DnaJ in protein refolding | 176, 272, 284 | None | NA | Yes | Yes | Yes | Yes |
| HPDb | Stimulates Hsp70 ATPase | 284 | None | NA | Yes | Yes | Yes | Yes |
| Zn finger (CXXCXGXG)b | Interacts with unfolded polypeptides and with denatured proteins | 176, 272 | None | NA | Yes | Yes | Yes | Yes |
| C terminus | Polypeptide (substrate) binding; possibly aids Zn fingers interact with protein substrates | 176, 272 | None | NA | Yes | Yes | Yes | Yes |
| HDELb | ER retention signal | 34, 107 | None | NA | No | No | No | No |
| CAAXb | Prenylation | 34, 35 | CaaX box | 34, 35 | No | No | No | No |
a
Abbreviations: NA, not applicable; ER, endoplasmic reticulum.
b
Amino acid single-letter symbols (X, any amino acid).
c
References are given in parentheses. Accession numbers are the same as in Table 6.