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. 1981 Jan;145(1):647–650. doi: 10.1128/jb.145.1.647-650.1981

Use of cir-lac operon fusions to study transcriptional regulation of the colicin Ia receptor in Escherichia coli K-12.

P L Worsham, J Konisky
PMCID: PMC217319  PMID: 7007329

Abstract

We describe cir-lac operon fusions constructed by using phage Mu d(Apr lac). Expression of beta-galactosidase in these fusion strains is analogous to known regulatory properties of cir gene expression. It is concluded that the observed regulation by iron of the cir gene is under transcriptional control.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun V., Hancock R. E., Hantke K., Hartmann A. Functional organization of the outer membrane of escherichia coli: phage and colicin receptors as components of iron uptake systems. J Supramol Struct. 1976;5(1):37–58. doi: 10.1002/jss.400050105. [DOI] [PubMed] [Google Scholar]
  2. Cardelli J., Konisky J. Isolation and characterization of an Escherichia coli mutant tolerant to colicins Ia and Ib. J Bacteriol. 1974 Aug;119(2):379–385. doi: 10.1128/jb.119.2.379-385.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Casadaban M. J., Cohen S. N. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. doi: 10.1073/pnas.76.9.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Casadaban M. J. Regulation of the regulatory gene for the arabinose pathway, araC. J Mol Biol. 1976 Jul 5;104(3):557–566. doi: 10.1016/0022-2836(76)90120-0. [DOI] [PubMed] [Google Scholar]
  5. Davies J. K., Reeves P. Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group B. J Bacteriol. 1975 Jul;123(1):96–101. doi: 10.1128/jb.123.1.96-101.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frost G. E., Rosenberg H. The inducible citrate-dependent iron transport system in Escherichia coli K12. Biochim Biophys Acta. 1973 Nov 30;330(1):90–101. doi: 10.1016/0005-2736(73)90287-3. [DOI] [PubMed] [Google Scholar]
  7. Hall M. N., Silhavy T. J. Transcriptional regulation of Escherichia coli K-12 major outer membrane protein 1b. J Bacteriol. 1979 Nov;140(2):342–350. doi: 10.1128/jb.140.2.342-350.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hollifield W. C., Jr, Neilands J. B. Ferric enterobactin transport system in Escherichia coli K-12. Extraction, assay, and specificity of the outer membrane receptor. Biochemistry. 1978 May 16;17(10):1922–1928. doi: 10.1021/bi00603a019. [DOI] [PubMed] [Google Scholar]
  9. Ichihara S., Mizushima S. Involvement of outer membrane proteins in enterochelin-mediated iron uptake in Escherichia coli. J Biochem. 1977 Mar;81(3):749–756. doi: 10.1093/oxfordjournals.jbchem.a131513. [DOI] [PubMed] [Google Scholar]
  10. Konisky J., Soucek S., Frick K., Davies J. K., Hammond C. Relationship between the transport of iron and the amount of specific colicin Ia membrane receptors in Escherichia coli. J Bacteriol. 1976 Jul;127(1):249–257. doi: 10.1128/jb.127.1.249-257.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McIntosh M. A., Earhart C. F. Coordinate regulation by iron of the synthesis of phenolate compounds and three outer membrane proteins in Escherichia coli. J Bacteriol. 1977 Jul;131(1):331–339. doi: 10.1128/jb.131.1.331-339.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murphy J. R., Michel J. L., Teng M. Evidence that the regulation of diphtheria toxin production is directed at the level of transcription. J Bacteriol. 1978 Aug;135(2):511–516. doi: 10.1128/jb.135.2.511-516.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pugsley A. P., Reeves P. The role of colicin receptors in the uptake of ferrienterochelin by Escherichia coli K-12. Biochem Biophys Res Commun. 1977 Feb 7;74(3):903–911. doi: 10.1016/0006-291x(77)91604-7. [DOI] [PubMed] [Google Scholar]
  14. SIMON E. H., TESSMAN I. THYMIDINE-REQUIRING MUTANTS OF PHAGE T4. Proc Natl Acad Sci U S A. 1963 Sep;50:526–532. doi: 10.1073/pnas.50.3.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Silhavy T. J., Shuman H. A., Beckwith J., Schwartz M. Use of gene fusions to study outer membrane protein localization in Escherichia coli. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5411–5415. doi: 10.1073/pnas.74.12.5411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Soucek S., Konisky J. Normal iron-enterochelin uptake in mutants lacking the colicin I outer membrane receptor protein of Escherichia coli. J Bacteriol. 1977 Jun;130(3):1399–1401. doi: 10.1128/jb.130.3.1399-1401.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Williams P. H. Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infect Immun. 1979 Dec;26(3):925–932. doi: 10.1128/iai.26.3.925-932.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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