Table 1.
Analysis of B cell hybridomas from RS−/− and RS+/+ mice for λ 1–3 secretion, κ/λ 1–3 protein coproduction, and RS recombination.
| a Fusion number | Mouse genotype | λ1–3(%) | λ+/ #screened | λ+/κ+ | λ+/κ | Total λ+ frequency | RS status of λ+ hybrids #rearranged / #tested | bRS recombination in λ+ hybridomas |
|---|---|---|---|---|---|---|---|---|
| 1 | RS+/+ | 7.4 | 2/27 | 0 | 2 | 9 % (22/244) | 1/1 | 100% (8/8) |
| 2 | 9 | 20/217 | 0 | 20 | 7/7 | |||
| 3 | RS−/− | 2.2 | 4/180 | 0 | 4 | 2.8% (17/603) | 0/4 | 0% (0/9) |
| 4 | 5 | 4/80 | 0 | 4 | 0/3 | |||
| 5 | 5 | 3/61 | 0 | 3 | 0/2 | |||
| 6 | 1 | 1/92 | 0 | 1 | ||||
| 7 | 2.6 | 5/190 | 0 | 5 |
a
Each line represents a single fusion experiment using spleen cells from a different individual mouse. Hybridoma clones were screened for κ and λ1–3immunoglobulin production as indicated in Experimental Procedures.
b
RS rearrangement status was assessed by PCR assay and genomic southern blotting. Hybridomas were scored positive if at least one RS rearrangement was detected.